N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid, also recognized as BES, finds frequent use across various laboratories and industrial setups. This compound offers a strong buffering capacity, keeping pH levels stable during complex chemical reactions, especially in biological and biochemical applications. Many researchers reach for BES when they need a buffer that stays reliable, even in fluctuating conditions. This reliability stems from its unique molecular structure and the way it interacts with other materials.
The molecular formula for BES comes out as C6H15NO5S, and it has a molecular weight of about 213.25 g/mol. Its core contains ethane rings connected to both sulfonic and amino groups, which creates an environment capable of both accepting and donating protons. This dual action stabilizes pH in many research environments. These chemical properties explain why BES usually shows up as a strong candidate in protein and enzyme studies. For those visualizing it in their laboratory, BES appears as solid, crystalline flakes or powders, and sometimes in small pearls or granules. Sometimes suppliers offer BES in solution form for easy handling and faster preparation.
BES gives off a white to off-white appearance, standing out in a range of solid forms. The density lands around 1.2 g/cm³, which makes it a bit lighter than other buffering agents. The compound does not have much of an odor, something researchers appreciate during long hours in the lab. It dissolves well in water, forming a clear, almost colorless solution, which helps when clarity matters in experiments. Storage rarely presents trouble, as BES in both flakes and powders resists moisture fairly well when kept sealed, and it doesn’t turn sticky or clump easily under normal room conditions. Liquids based on BES stay stable too, but always demand tightly capped containers.
BES acts as a zwitterion, balancing electrical charge within its structure. This gives it a steady behavior in both acidic and basic settings. Its useful pH range falls between 6.4 and 7.8, fitting for many biochemical tasks. The purity grade of BES available ranges from industrial to research-grade, depending on intended use; research often demands more than 99% purity. Solubility in water reaches beyond 50 grams per liter, so even concentrated solutions won’t leave chunky residues. Usually, the shelf life stretches over several years as long as users avoid introducing contamination. The HS Code for BES usually fits under 2922.19, which covers amino-compounds with sulfonic groups, something importers and exporters keep in mind to ensure smooth customs processing.
Most users find BES relatively safe when used as directed. This acid does not carry a strong risk of toxicity, but as with any chemical raw material, caution pays off. Wearing gloves and goggles makes sense in case dust or solution splashes onto skin or eyes. The material safety data sheet often classifies BES as non-hazardous, though any ingestion or inhalation over long periods should be avoided. In case of spills, sweeping up the solid or diluting the solution with water minimizes risk, and proper disposal sparingly strains waste systems. In my own years testing pH buffers, laboratory colleagues never showed serious reactions, but nobody ever skipped the extra rinse after direct contact. Safety showers, eyewash stations, and labeled containers remain basic requirements in every setting dealing with BES or any similar chemical.
BES plays a clear role in creating controlled environments in cell culture, protein purification, and enzyme assays. Its stability attracts those aiming for repeatable results. Manufacturing facilities that synthesize diagnostic kits, molecular biology reagents, and advanced materials value BES for its consistency and ability to withstand a wide range of conditions. Its presence in finished products barely registers, but it shapes results quietly in the background. Global trade in BES remains brisk, with reliable supply chains leading researchers and producers to count on steady delivery of this raw material, governed by transparent labeling under HS Codes and by adherence to national safety standards.
Beyond workbench safety, the broader concern involves safe transit and proper labeling. Supply chain missteps do happen, especially when international paperwork misses the right chemical code or hazard category. Clear communication between buyer and supplier helps prevent shipment delays or regulatory scrutiny. Another issue comes up during manufacturing, where dust can pose inhalation risk. Facility upgrades, like better ventilation and localized exhaust systems, cut down on accidental exposure. In research spaces, routine staff training in handling procedures, waste neutralization, and emergency response limits health hazards. As global use of BES expands, manufacturers continue refining both the purity and packaging, making the compound safer and more convenient to handle, both on a large scale and at the bench.
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid sits among the key chemical raw materials that help power scientific progress worldwide. Spin up any protein or DNA analysis workflow, and chances are high that BES might play a part in stabilizing pH or ensuring consistency across samples. Those working with BES appreciate its solid performance and manageable risk profile. Keeping safety, labeling, and communication central means this material delivers on its promise across industries, ensuring both progress and peace of mind for teams that rely on its steady, reliable profile.
